Cells need to be > 2M/mL and > 50% viability (> 70% is preferred).
Run samples of cells on Vi–CELL XR using the desired Cell Types. Before proceeding the concentration measurements of instruments need to be within 5% of each other. (Alternative bead concentrations can be utilized as long as the concentration is determined using another particle counter such as a Multisizer Coulter Counter). Instrument performance was then verified using 1M beads/mL BEC concentration controls (Catalog number 175478) on Vi–CELL XR and Vi–CELL BLU instruments. The Vi–CELL XR and Vi–CELL BLU instruments were first baselined using manufacturer recommended standard beads and protocols. Replicate samples were then run using the new Cell Type to confirm the results. The Vi–CELL BLU data were reanalyzed, adjusting the Cell Type parameters as necessary, until a new Cell Type provided a Cell Concentration match within +/–5%* and Viable cell density levels within +/–2.5%* of the Vi-CELL XR results. Duplicate samples were also run on Vi–CELL BLU instruments using the system default Cell Type parameters. #Beckman coulter vicell series
To demonstrate this, a series of different types of cells were run on the Vi–CELL XR using typical Cell Type parameters for the cells used. However, for many standard types of cells it should be possible to match the results between machines to acceptable levels.
Complete matching may not be possible in all cases as the performance characteristics between the two instrument are different. With this consideration in mind we have designed the Vi–CELL BLU with the flexibility to adjust the measurement parameters to match results obtained on an existing Vi–CELL XR system. When a new instrument technology is introduced it may be necessary to revalidate existing protocols on the new platforms. Plate Loader Options for the CytoFLEX Platform.CytoFLEX Violet-Blue-Red Series Upgrades.Labware for Liquid Handling Instruments.Lysing, Fixative, and Permeabilizating Reagents.RESOURCE Contract Manufacturing Services.Search Conjugated Antibodies, Antibody Cocktails.HIAC 9703+ Pharmaceutical Particle Counter.HIAC 8011+ Industrial Laboratory System.
Echo 650 Series Acoustic Liquid Handlers. Navios EX – Powerful, Dependable Flow Cytometer. Cell Counters, Sizers and Media Analyzers. Air Particle Counters for Cleanroom and Environmental Monitoring. Need a certificate of analysis (COA)? Use the search bar at the top of the page and search your lot number. This issue is corrected if a decontamination cycle is performed and the Beckman Coulter Vi-CELL ™ Reagent Pak is subsequently used on the system. Our in-house testing has demonstrated that using Non-Beckman Coulter reagents with the Vi-CELL ™ leads to gradual darkening of the flow cells which lowers the background intensity and generates a light intensity error message. Using Non-Beckman Coulter Vi-CELL ™ reagents may result in frequent light intensity errors and clogging of the fluid lines, creating unnecessary Service visits. Validated reagent with appropriate cleaning and disinfectant solutionsĭon’t put your experiments at risk, always use Beckman Coulter reagents, a company you know and trust. So, let us illustrate the value and peace of mind that you will receive when you purchase Vi-CELL ™ Reagents from Beckman Coulter Life Sciences. Trust is an integral component of any relationship. 
ANATEL A1000 TOC Analyzer for Ultrapure Water.
0 kommentar(er)
